Tag Archives: dermatology mosiac

<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-275">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-274">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-273">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-272">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-271">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-270">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-269">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-268">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-267">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-266">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-265">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-264">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-263">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-262">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-259">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-258">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-256">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-255">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-254">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-252">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-251">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-249">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-248">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-247">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-245">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-244">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-243">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-242">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-239">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-238">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-237">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-236">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-235">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-234">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-233">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-232">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-225">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-224">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-222">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-221">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-220">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-219">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-218">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-214">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-211">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-210">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-209">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-208">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-207">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-206">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-205">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-204">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-203">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-202">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-201">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-200">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-199">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-198">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-197">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-196">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-194">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-193">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-191">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-190">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-189">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-188">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-187">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-186">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-185">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-184">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-182">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-181">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-180">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-178">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-177">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-176">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-175">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-174">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-173">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-172">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-171">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-170">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-169">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-168">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-167">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-166">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-162">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-160">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-159">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-158">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-157">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-156">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-155">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-154">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-152">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-151">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-150">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-149">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-148">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-147">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-146">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-145">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-144">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-143">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-142">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-141">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-140">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-139">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-138">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-137">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-136">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-135">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-134">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-133">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-132">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-131">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-130">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-129">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-128">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-127">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-126">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-125">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-124">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-123">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-121">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-117">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-116">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-101">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-100">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-99">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-98">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-97">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-96">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-95">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-94">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-93">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-92">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-87">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-86">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-85">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-84">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-78">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-77">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-76">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-49">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-38">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-34">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-33">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-30">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-29">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-28">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-25">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-19">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-5">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-4">span class="explanatory-dictionary-highlight" data-definition="explanatory-dictionary-definition-3">div class="row">
Bullous pemphigoid (BP) is an autoimmune blistering skin disease. Auto-antibodies to BP180 and BP230 can be detected by indirect immunofluorescence (IIF) on different substrates (oesophagus, salt-split-skin, BP180-antigen dots, BP230-transfected cells) and ELISA. Here, we compared test characteristics of these test systems. We analyzed sera from BP patients (n=60) in whom the clinical diagnosis had been confirmed histopathologically. The control cohort comprised sera from patients with other autoimmune-associated (n=22) or inflammatory (n=35) skin diseases. All samples were tested by IIF (EUROIMMUN™ Dermatology Mosaic) and ELISA (EUROIMMUN and MBL). Anti-BP180 is best detected with BP180-antigen dots by IIF (sensitivity: 88%; specificity: 97%). As compared to IIF, the differences with both BP180 ELISA techniques are small though. Likelihood ratios (LRs) for positive and negative test results are >10 and between 0.1 and 0.2, respectively, for all test systems. Detection of anti-BP230 is highly variable (sensitivity range 38-60%; specificity range 83-98%). Only the IIF test reveals a LR for positive test results >10. Since the LRs for a negative test are all ~0.5, negative test results for anti-BP230 antibodies do not help to exclude BP. In conclusion, the multi-parameter IIF test reveals a good diagnostic performance in BP. Since this test simultaneously allows for the detection of anti-Dsg1 and anti-Dsg3 antibodies, involved in pemphigus foliaceus and vulgaris, a single test-incubation may be sufficient to differentiate between the most frequent autoimmune blistering diseases.

Copyright © 2012 Elsevier B.V. All rights reserved.

 

Source: http://www.ncbi.nlm.nih.gov/pubmed/22580378?dopt=Abstract